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KMID : 0363620080290010106
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 1 p.106 ~ p.116
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ROS-, RNS-Scavenging and Anti-inflammatory Activities of Mori Fructus
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Park Soon-Jae
Jung Ji-Chun
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Abstract
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Objectives: Peroxynitrite (ONOO-), superoxide anion radical ( O2-) and nitric oxide (NO) are cytotoxic because
they can oxidize several cellular components such as proteins, lipids and DNA. They have been implicated in the aging processes, and age-related diseases such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis.
The aim of this study was to investigate the ONOO-, NO, and O2 - scavenging and anti-inflammatory activities of Mori Fructus in ob/ob mice.
Methods: Mice were grouped and treated for 5 weeks as follows. Both the normal lean (C57/BL6J black mice)
and control obese (ob/ob mice) groups received the standard chow. The experimental groups were fed a diet of chow
supplemented with 7.5, 15 and 30 mg Mori Fructus per 1 kg of body weight for 14 days. For this study, the fluorescent probes, namely 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Western blotting was performed using anti-phospho IκB-α, anti-IKK-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS respectively.
Results: Mori Fructus inhibited the generation of ONOO-, NO and O2 - in the lipopolysaccharide (LPS)-treated
mouse kidney postmitochondria in vitro. The generation of ONOO-, NO and O2- were inhibited in the Mori Fructus-administered ob/ob mice groups. The GSH/GSSG ratio decreased in the ob/ob mice, whereas they improved
in the Mori Fructus-administered groups. Mori Fructus inhibited the expression of phospho IκB-α, IKK-α, COX-2,
iNOS genes, and thereby the activation of NF-κB.
Conclusions: These results suggest that Mori Fructus is an effective ONOO-, O2 - and NO scavenger, and therefore it might be a potential therapeutic drug against the inflammation process and inflammation-related diseases.
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KEYWORD
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Mori Fructus, peroxynitrite, nitric oxide, reactive oxygen species, NF-κB, COX-2, iNOS
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